◆A sequence of DNA that reads the same whether read from 5’ -> 3’ or from 3’ -> 5’direction is a palindromic sequence. ◆These are the sites for action of restriction enzymes. Almost all...
(a) Plasmid DNA and Chromosomal DNA (b) RNA and DNA (c) Exonuclease and Endonuclease A) Plasmid DNA ◆Plasmid DNA Chromosomal DNA It is an extra chromosomal DNA molecule found...
(a) PCR (b) Restriction enzymes and DNA (c) Chitinase Solution: 1.PCR ◆PCR or polymerase chain reaction is a technique to amplify a gene or a DNA fragment in...
DNA CONSTRUCT ●A collective DNA construct is introduced into the cell where the reporter gene is used as a selectable marker to discover the successful uptake of foreign genes. ● Example...
◆Meiosis is a process involving a reduction in the quantity of genetic material . ◆ type of cell division- It occurs in two phases are - 1.meiosis I 2.meiosis II ◆ pachytene event...
◆Stirred tank bioreactors are developed for a large-scale production of biotechnology products but the shake flask method is used for a small-scale production of biotechnological products carried...
● Eukaryotic cells do not have restriction endonucleases as the DNA of eukaryotes cells is highly methylated by methylase – a modification enzyme. ● Methylation safeguards the DNA from the...
◆ Molar concentration of human being DNA in a human is : ◆6.023 x 1023 x Total number of chromosomes ◆6.023 x 1023 x 46 ◆2.77 x 1023 moles ◆ 2.77 x 1023 moles is the molar concentration of DNA...
●Compared to DNA molecules, enzymes are smaller in size. ● DNA comprises genetic material, which is essential for the normal development and functioning of living cells. ●A DNA molecule...
●Steps in the formation of recombinant DNA by action of restriction endonuclease enzyme written as – EcoRI ●It can be diagrammatically represent as -
●Recombinant proteins used in medical practice which are obtained from the recombinant DNA technique . ●In this technology a particular gene is transferred from one organism to...