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Home » Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because : (1) mutated gene partially appears on a photographic film (2) mutated gene completely and clearly appears on a photographic film (3) mutated gene does not appear on a photographic film as the probe has no complementarity with it (4) mutated gene does not appear on photographic film as the probe has complementarity with it
Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because : (1) mutated gene partially appears on a photographic film (2) mutated gene completely and clearly appears on a photographic film (3) mutated gene does not appear on a photographic film as the probe has no complementarity with it (4) mutated gene does not appear on photographic film as the probe has complementarity with it
biology
Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because : (1) mutated gene partially appears on a photographic film (2) mutated gene completely and clearly appears on a photographic film (3) mutated gene does not appear on a photographic film as the probe has no complementarity with it (4) mutated gene does not appear on photographic film as the probe has complementarity with it

Correct option:(3)mutated gene does not appear on a photographic film as the probe has no complementarity with it
Solution:

  • Autoradiography allows the detection/localisation of radioactive isotope within a biological sample.
  • Probe is a radiolabelled ss DNA or ss RNA depending on the technique.
  • To identify the mutated gene probe is allowed to hybridise to its complementary DNA in a clone of cells followed by
    detection using autoradiography.
  • Mutated gene will not appear on the photographic film because the probe does not have complementarity with the
    mutated gene.
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